Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging.
نویسندگان
چکیده
Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.
منابع مشابه
A New Reporter Gene Technology: Opportunities and Perspectives
The paper summarizes the current status of the reporter gene technology and their basics. Reporter gene technology is widely used to monitor cellular events associated with gene expression and signal transduction. Based upon the splicing of transcriptional control elements to a variety of reporter genes, it “reports” the effects of a cascade of signaling events on gene expression inside cells. ...
متن کاملIn Vivo Long-Term Tracking of Neural Stem Cells Transplanted into an Acute Ischemic Stroke model with Reporter Gene-Based Bimodal MR and Optical Imaging
Transplantation of neural stem cells (NSCs) is emerging as a new therapeutic approach for stroke. Real-time imaging of transplanted NSCs is essential for successful cell delivery, safety monitoring, tracking cell fate and function, and understanding the interactions of transplanted cells with the host environment. Magnetic resonance imaging (MRI) of magnetic nanoparticle-labeled cells has been ...
متن کاملIn vivo imaging of tumor transduced with bimodal lentiviral vector encoding human ferritin and green fluorescent protein on a 1.5T clinical magnetic resonance scanner.
A combination of reporter genes for magnetic resonance imaging (MRI) and optical imaging can provide an additional level of noninvasive and quantitative information about biological processes occurring in deep tissues. We developed a bimodal lentiviral vector to monitor deep tissue events using MRI to detect myc-tagged human ferritin heavy chain (myc-hFTH) expression and fluorescence imaging to...
متن کاملivo Imaging of Tumor Transduced with Bimodal Lentiviral tor Encoding Human Ferritin and Green Fluorescent R tein on a 1.5T Clinical Magnetic Resonance Scanner
Downlo mbination of reporter genes for magnetic resonance imaging (MRI) and optical imaging can provide an nal level of noninvasive and quantitative information about biological processes occurring in deep tise developed a bimodal lentiviral vector to monitor deep tissue events using MRI to detect myc-tagged ferritin heavy chain (myc-hFTH) expression and fluorescence imaging to detect green flu...
متن کاملAnalytical determination of the chemical exchange saturation transfer (CEST) contrast in molecular magnetic resonance imaging
Magnetic resonance based on molecular imaging allows tracing contrast agents thereby facilitating early diagnosis of diseases in a non-invasive fashion that enhances the soft tissue with high spatial resolution. Recently, the exchange of protons between the contrast agent and water, known as the chemical exchange saturation transfer (CEST) effect, has been measured by applying a suitable pulse ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 51 23 شماره
صفحات -
تاریخ انتشار 2015